Review





Similar Products

tak1  (MedChemExpress)
94
MedChemExpress tak1
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
Tak1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/Phospho-TAK1+(Ser439)+Antibody/pmc13386909-116-31-34
Average 94 stars, based on 1 article reviews
tak1 - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier
tak1  (ABclonal Biotechnology)
94
ABclonal Biotechnology tak1
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
Tak1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/TAK1+Rabbit+mAb/pm42892647-235-88-89
Average 94 stars, based on 1 article reviews
tak1 - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier
tak1  (Proteintech)
95
Proteintech tak1
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
Tak1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/TAK1+Antibody/pm42772408-125-15-17
Average 95 stars, based on 1 article reviews
tak1 - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier
p tak1  (ABclonal Biotechnology)
94
ABclonal Biotechnology p tak1
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
P Tak1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/Phospho-TAK1-S412+Rabbit+pAb/pm41678235-100-46-49
Average 94 stars, based on 1 article reviews
p tak1 - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier
anti tak1  (Proteintech)
93
Proteintech anti tak1
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
Anti Tak1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/MAP3K7+Monoclonal+antibody/pmc12999352-19-0-2
Average 93 stars, based on 1 article reviews
anti tak1 - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
p tak1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc p tak1
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
P Tak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/Phospho-TAK1+(Thr184%2F187)+Rabbit+mAb/pm41922912-78-5-6
Average 95 stars, based on 1 article reviews
p tak1 - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier
mouse anti phospho tak1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc mouse anti phospho tak1
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
Mouse Anti Phospho Tak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/TAK1+Antibody/pmc12945651-90-24-27
Average 96 stars, based on 1 article reviews
mouse anti phospho tak1 - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
mouse anti tak1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc mouse anti tak1
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
Mouse Anti Tak1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/TAK1+Antibody/pmc12945651-90-32-35
Average 96 stars, based on 1 article reviews
mouse anti tak1 - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
tak1 a19077  (ABclonal Biotechnology)
94
ABclonal Biotechnology tak1 a19077
PRRSV nsp1 activates JNK and P38 signaling pathways through <t>TAK1</t> and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.
Tak1 A19077, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tak1/TAK1+Rabbit+mAb/pm41888097-231-3-11
Average 94 stars, based on 1 article reviews
tak1 a19077 - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier

Image Search Results


PRRSV nsp1 activates JNK and P38 signaling pathways through TAK1 and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.

Journal: Journal of Virology

Article Title: Highly pathogenic porcine reproductive and respiratory syndrome virus nonstructural protein 1 interacts with TRAF6 to activate the TAK1/p38/JNK/AP-1 signaling and induce IL-1β

doi: 10.1128/jvi.00428-26

Figure Lengend Snippet: PRRSV nsp1 activates JNK and P38 signaling pathways through TAK1 and TRAF6. ( A–C ) CRL-2843 cells were pretreated with the TAK1 inhibitor (5Z-7-Oxozeaenol), TRAF6 inhibitor (HY-120934), or IRAK inhibitor (HY-13329) at different doses, and 1 h later, the cells were transfected with nsp1 plasmid (1 μg). The total RNAs were extracted at 24 h post-transfection for IL-1β mRNA analysis by qPCR. ( D and F ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. Cells were collected 12, 24, and 48 h after transfection, and the mRNA level of TAK1 or TRAF6 was analyzed by qPCR. ( E and G ) CRL-2843 cells were transfected with TAK1 or TRAF6 siRNA (60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). Cells were collected at 24 h, and the mRNA level of IL-1β was analyzed by qPCR. ( H ) CRL-2843 cells were transfected with TRAF6 siRNA (30 or 60 nM), with NC as the control. The medium was changed at 12 h, followed by transfection with the nsp1 plasmid (1 μg). The cells were harvested at 24 h for western blot analysis. Differences were evaluated by Student's t- test. *, P < 0.05; **, P < 0.01; ns, not significant.

Article Snippet: Membranes were blocked with 5% skim milk in PBS for 1 h at room temperature, followed by incubation overnight at 4°C with antibodies against p-c-Jun, c-Jun, p-p38, p38, p-JNK, JNK, p-TAK1, TAK1, and TRAF6 (MCE, USA).

Techniques: Protein-Protein interactions, Transfection, Plasmid Preparation, Control, Western Blot

Model for HP-PRRSV nsp1 to induce IL-1β production. After HP-PRRSV infection, the viral protein nsp1 induces the expression of IL-1β via interacting with TRAF6 to activate the TAK1/p38/JNK/AP-1 signaling pathway, in which nsp1 R308 and R375 are the key amino acids for nsp1 to induce IL-1β production.

Journal: Journal of Virology

Article Title: Highly pathogenic porcine reproductive and respiratory syndrome virus nonstructural protein 1 interacts with TRAF6 to activate the TAK1/p38/JNK/AP-1 signaling and induce IL-1β

doi: 10.1128/jvi.00428-26

Figure Lengend Snippet: Model for HP-PRRSV nsp1 to induce IL-1β production. After HP-PRRSV infection, the viral protein nsp1 induces the expression of IL-1β via interacting with TRAF6 to activate the TAK1/p38/JNK/AP-1 signaling pathway, in which nsp1 R308 and R375 are the key amino acids for nsp1 to induce IL-1β production.

Article Snippet: Membranes were blocked with 5% skim milk in PBS for 1 h at room temperature, followed by incubation overnight at 4°C with antibodies against p-c-Jun, c-Jun, p-p38, p38, p-JNK, JNK, p-TAK1, TAK1, and TRAF6 (MCE, USA).

Techniques: Infection, Expressing